Chemical composition, antioxidant, and antibacterial activity of Ruta graveolens (Rutaceae)

Abstract. Saeed YS, Ali JF, Mohammed MA. 2023. Chemical composition, antioxidant, and antibacterial activity of Ruta graveolens (Rutaceae). Biodiversitas 24: 3162-3168. The present study was carried out to investigate the antioxidant activities phenolic components isolated from leaves L. compounds were extracted in different organic solvents (hexane, ethanol, ethyl acetate) analyzed by column chromatography, thin-layer high-performance liquid chromatography. Three discrete fractions collected subsequently examined: one acetate extraction (I) two ethanolic (II III). No hexane obtained. In particular, HPLC analysis which contained most bioactive compounds. major ethanol extracts catechol coumarin (1.104966 16.612227 mg/kg), followed hydroquinone (0.144731 1.90781 mg/Kg), whereas, resorcinol (14.78298 21.75253 mg/kg). extract had highest level activity, other effects at levels; because three fractions' chemicals have varied redox characteristics, their impacts vary strength. inhibition zones (mm) Bacillus cereus Staphylococcus aureus 24 ?g/mL for II I 23 22 mm, respectively. At ?g/mL, Escherichia coli Proteus vulgaris 21 20 Fraction did not show 1.5 µg/mL. Finally, study's results suggest that using R. may be a natural source antioxidants anti-infectious agents.